Fluorescence

Iridian specializes in supplying high performance fluorescence filters to some of the leading OEMs of analytical optical instruments.  In fluorescence spectroscopy, light from a broadband light source or a laser is incident upon a sample which may then emit a weak fluorescence signal.   A Fluorescence filter set usually consists of an excitation filter, dichroic filter and emission filter combined in a filter cube. Excitation filters are used to ensure that only the desired wavelength is incident upon the sample while an emission filter is used to pass the fluorescence signal and reject the excitation light and any undesirable light.  A dichroic filter is typically used at an angle of incidence of 45° to reflect excitation light to the sample and transmit the emitted signal to the detector.  The excitation, emission and dichroic filter are commonly used in a set to maximize the desired signal and minimize any undesired signal.

 

 

 

Fluorescence spectroscopy is widely used in many biomedical applications including Confocal Microscopy and Flow Cytometry.  In addition, Iridian also deals directly with users for upgrades or replacement fluorescence filter sets.  Iridian’s fluorescence filters have high transmittance, low ripple and deep blocking.  We offer custom design and manufacture where required.

Further information on Iridian’s fluorescence filters:

 

 

Filter Sets

 

Fluorescence Filters (Single Band)

 

Fluorescence Filters (Multiple Bands)

 

Custom Fluorescence Filter Solutions

 

 

Additional Resources

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